Composite

Part:BBa_K2692005:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge_HS   (2018-10-08)


Major T4 Capsid Protein gp24


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1649
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1650
    Illegal PstI site found at 1664
    Illegal NotI site found at 1657
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 825
    Illegal BamHI site found at 1320
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1650
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1650
    Illegal PstI site found at 1664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All parts are optimized for use in E.coli .

Source

https://www.uniprot.org/uniprot/P04535

References

[1] Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391